N a previous study, we assessed the possible of sivelestat, a competitive inhibitor of human neutrophil elastase (NE) (8), within the protection against acute pancreatitis-associated lung injury within a rat model (9). As an extension in the analyses in ourCorrespondence to: Dr Li Chen, Department of Surgery, ZhejiangUniversity College of Medicine, Second Affiliated Hospital, 88 Jiefang Street, Hangzhou, Zhejiang 310009, P.R. China E-mail: [email protected]*Contributed equallyKey words: acute pancreatitis, neutrophil elastase, sivelestat,renoprotectionWANG et al: RENOPROTECTIVE ACTIVITY OF SIVELESTATprevious study, the present study aimed to evaluate the capability of sivelestat to guard against renal injury in acute pancreatitis in rats. Supplies and solutions Animals, experimental style and specimen collection. Because this study was an extension of a earlier study from our group, the animals and their allocation, at the same time because the methods of pancreatitis induction and sivelestat treatment, were precisely the same as described in our prior study (9). In summary, adult male Sprague-Dawley rats were randomized into the following groups: i) the experimental acute pancreatitis (EAP) group in which rats were induced to create acute pancreatitis by the administration of sodium taurocholate by way of laparotomy beneath anesthesia; ii) the EAP plus sivelestat therapy group in which rats have been injected with two mg/kg sivelestat by way of the superficial dorsal vein from the penis promptly following EAP induction; and iii) the handle group in which a sham laparotomy was performed.3-Bromo-1-naphthoic acid Price At six, 12 and 24 h soon after sivelestat or car therapy, six rats from every group were sacrificed by depletion.2378-02-1 Data Sheet Right away prior to and after animal sacrifice, blood samples had been collected through cardiac puncture and serum was ready. Tissue blocks of your kidneys and pancreas have been excised and fixed in 10 formalin or snapfrozen in liquid nitrogen The study protocol was authorized by the Institutional Overview Board of Zhejiang University (Hangzhou, China). Histological examination. Formalin-fixed kidney tissue blocks were dehydrated, embedded in paraffin, sectioned at 5 and stained with hematoxylin and eosin (H E). Abnormalities, which includes vacuolization from the tubular epithelial lining within the subcapsular region, patchy areas of hemorrhage within the interstitium and necrosis in the epithelial lining on the tubules towards the medullary region had been assessed by two independent pathologists who were blind to the study design and the specimen identities. Renal function test. Serum levels of blood urea nitrogen (BUN) and creatinine (CR) were determined utilizing regular laboratory solutions. Measurement of serum tumor necrosis factor- (TNF- ).PMID:32472497 Serum levels of TNF- had been determined using an immunoassay kit (Biosource, Grand Island, NY, USA) following the manufacturer’s directions. Measurement of NE activity and cytokine-induced neutrophil chemoattractant-1 (CINC-1) level in renal tissue. Tissue homogenate was ready from frozen renal specimens applying the strategy described for the homogenate preparation of lung tissue in our preceding study (9). NE activity was determined spectrophotometrically using a chromogenic substrate. Levels of CINC-1 have been measured with a sandwich enzyme-linked immunosorbent assay (ELISA) kit (R D Systems, Minneapolis, MN, USA). Statistical analysis. Information are expressed as arithmetic implies ?standard deviation (SD) and analyzed with one-wayTable I. Serum levels of BUN (mmol/l) in t.