Ti-CD103. Both frequency of CD103+ CD8 T cells and MFI are presented (n=5). (E) Tumor volumes measured over time in B16 melanoma model treated with IC, anti-CD103, anti-LAP or combined anti-CD103+anti-LAP (n=5). (F) Percent survival in sufferers with comparatively higher or low mRNA expression of ITGAE (CD103) according to z score (details are as in Fig. 1N). Final results for low grade glioma and glioblastoma are shown. Error bars, imply .e.m. Two-way ANOVA (A, C, and E) and two-tailed t-test (B and D) have been made use of for p value calculations. P values for the last time points are shown.Sci Immunol. Author manuscript; obtainable in PMC 2017 October 26.Gabriely et al.PageAuthor Manuscript Author Manuscript Author ManuscriptFigure 7. Anti-LAP remedy combined with antigen certain vaccination enhances tumor immunotherapy and improves immune memory(A) Study style.Price of 1867923-49-6 (B) Percentage of tumor free mice (n=5; log-rank test).1228561-86-1 Chemscene Information are representative of two independent experiments.PMID:25105126 (C, D) Accumulation of memory-like CD8+ T cells, according to IL7R and KLRG1 expression in anti-LAP treated mice. Representative flow cytometry dot plots (C) and quantification (D) are shown (n=4 (anti-LAP and IC) and n=3 (not vaccinated)). (E) Percentage of memory-like CD8+ T cells, according to CCR7 and CD62L expression in anti-LAP treated mice (n=4 (anti-LAP and IC) and n=3 (not vaccinated)). (F, G) Frequency of memory-like CD8+ T cells, depending on IL7R and CD62L expression in anti-LAP treated mice. Representative flow cytometry dot plots (F) and quantification (G) are shown (n=4 (anti-LAP and IC) and n=3 (not vaccinated)). (H)Author ManuscriptSci Immunol. Author manuscript; out there in PMC 2017 October 26.Gabriely et al.PagePercentage of IL7R+CD103- CD8 T cells (n=4 (anti-LAP and IC) and n=3 (not vaccinated)). (I) Study style. (J) Representative MRI photos of mice treated with either IC or anti-LAP. (K) Survival curves of mice pre-vaccinated with DC-OVA, implanted with intracranial GBM and treated with anti-LAP (n=5; log-rank test). (L) Frequencies of OVATCR-specific CD8+ T cells (stained with H-2Kb OVA Pentamer) in mice treated with antiLAP. (M, N) Accumulation of memory-like CD8+ T cells in anti-LAP treated mice. Representative flow cytometry dot plots (M) and quantification (N) are shown (n=4; twotailed t-test). (O, P) Mice vaccinated with DC-OVA and treated by anti-LAP develop immune memory. (O) Study design. (P) Accumulation of memory-like IL7R+CD44+ CD8 T cells in anti-LAP treated mice by flow cytometry (n=4). Error bars, imply .e.m. One-way ANOVA (D, E, G, H, and P) was utilised for p value calculations.Author Manuscript Author Manuscript Author Manuscript Author ManuscriptSci Immunol. Author manuscript; out there in PMC 2017 October 26.
Saudi Journal of Biological Sciences (2015) 22, 532King Saud UniversitySaudi Journal of Biological Scienceswww.ksu.edu.sa www.sciencedirect.comORIGINAL ARTICLEAnti-influenza (H1N1) possible of leaf and stem bark extracts of selected medicinal plants of South IndiaGansukh Enkhtaivan a,1, K.M. Maria John a,1, Muniappan Ayyanar b, Thangavel Sekar c, Ki-Joun Jin a, Doo Hwan Kim a,*Department of Bio-resources and Food Science, Konkuk University, Seoul 143-701, South Korea Division of Botany Microbiology, AVVM Sri Pushpam College (Autonomous), Poondi, Thanjavur District, Tamil Nadu 613503, India c Department of Botany, Pachaiyappa’s College, Chennai 600030, Indiab aReceived two October 2014; revised 9 January 2015; accepted 12 January 2015 Accessible on the internet 20 Ja.