In Ayurvedic program of medicine, the leaves and roots are made use of inside the treatment of indolent ulcers (Kirtikar and Basu,) and diarrhea (Amresh et al.,). The plant is employed within the remedy of urinary tract infections since it really is regarded as antiseptic (Dandiya and Chopra,). Juice of C. pareira is provided in migraine and the plant includes a long history of use for inflammation of muscle tissues, snakebite, rheumatism, diarrhoea, dysentery and menstrual challenges. C. pariera is broadly employed in herbal medicine today as a diuretic, tonic also as to decrease fever and to relieve pain. It is generally employed for menstrual cramps, dysmenorrhoea, excessive bleeding and uterine hemorrhages, fibroid tumors, pre and postJournal of Clinical and Diagnostic Study. 2014 May, Vol8(five): HC01HCMETHODOLOGYCollection with the PlantThe roots of C.pareira were obtained from the forest of Tirupati, AP and had been identified and authenticated by Dr. Pramod Kumar, Pharmacognocist V.L. College of Pharmacy, Raichur, Karnataka, India.Preparation of ExtractRoots were thoroughly washed below fresh tap water and shade dried and powdered by utilizing a mechanical grinder. The preparationSuresh Babu Sayana et al., Evaluation of Diuretic Activity of Alcoholic Extract of Roots of Cissampelos Pareira in Albino Ratswww.jcdr.netof alcoholic extract of roots of C.pareira was carried out by using soxhletation within the Department of Pharmacology, V.L.College of Pharmacy, Raichur. About 200 g of root powder was taken into the soxhlet apparatus and extracted applying ethanol (95 ). The extraction approach was carried out for 18 20 h till the look of colourless solvent inside the side tube. The extract collected was dried by evaporating the solvents on a water bath maintained at 500C and percentage yield of alcoholic extract was recorded with respect towards the total quantity of powder applied for the extraction. Phytochemical evaluation for the extract was performed using regular procedures.kept at 21 .5 . The total volume of urine collected for 5 hr was measured in the finish. Throughout this period no food and water was produced accessible to animals. Many parameters like total urine volume and concentration of sodium, potassium and chloride within the urine had been measured and estimated respectively.Estimation of Urinary ElectrolytesUrine electrolytes (sodium, potassium and chloride) were determined by Ion Selective Electrode technique as described by the user instruction manual from the biochemical kits (Roche, Roche Diagnostics Pvt.Methyl 1H-1,2,3-triazole-4-carboxylate site Ltd, Gurgaon, Haryana.Methyl 4-aminothiazole-5-carboxylate site )Experimental AnimalsAlbino rats weighing in between 140200 g of either sex have been employed inside the study and have been obtained in the Central Animal Residence, V.PMID:23833812 L.College of Pharmacy, Raichur, Karnataka, India. The experimental protocol was approved by the Institutional Animal Ethical Committee and these animals had been made use of to evaluate the diuretic activity of alcoholic extract of roots of Cissampelos pareira. The animals had been maintained below common husbandry situations for an acclimatization period of 15 days just before performing the experiments. All rats have been housed in metallic cages 6 in every and temperature maintained at 222 .STATISTICAL ANALYSISExperimental outcomes have been expressed as imply SEM (n=6). Statistical analysis was performed with onewayANOVA followed by Dunnetts ttest.RESULTSThe alcoholic extract of roots of Cissampelos pareira was subjected to qualitative phytochemical tests to recognize the phytoconstituents and it revealed the presence of carbohydrates, alkaloids, sterols, phen.